FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9463408
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG268750.2839886011466482TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38050.039.97415545
GATCGGA11700.033.855471
ATCGGAA49350.033.1094131
TCTCGTA46300.032.8041240
CGTATGC46950.032.73319643
CTCGTAT46250.032.693841
TCGGAAG49700.032.6004072
ACACGTC48350.032.06038312
TCGTATG47750.031.94951242
CGTCTGA48750.031.38247315
TCACGAT48650.031.35581234
ATCTCGT48600.031.20453839
CGGAAGA50950.030.6964073
CATCACG50500.030.60843732
ACGTCTG51000.030.21852114
CACGTCT52650.029.5274313
GCACACG53850.029.45387510
CACGATC52500.029.05607835
ACGATCT52250.028.93811636
GTATGCC53300.028.9171344
Produced by FastQC (version 0.11.5)