FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8656552
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGC126120.14569311199193397TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19900.036.97445745
ATCGGAA20400.036.443751
CTCGTAT19900.036.40909643
AGTTCCG19800.036.13590633
ACACGTC20400.036.06405312
TCCGTAT17400.035.56030336
CGTCTGA20700.035.54179415
CCGTATC17450.035.45841237
TCTCGTA17650.035.31361442
GTTCCGT20100.035.2609534
TATCTCG17550.035.25779340
TTCCGTA20000.035.21225735
TCGGAAG21650.034.294782
TCGTATG21500.034.1182144
ATCTCGT19050.032.83593441
CGGAAGA22950.032.351973
ACGTCTG22950.032.1553414
CGTATCT19650.031.83202638
CACGTCT24000.030.93567713
GCACACG25650.028.85794810
Produced by FastQC (version 0.11.5)