FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16739481
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG13454738.037722316480421TruSeq Adapter, Index 7 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGCCG457000.2730072694607437TruSeq Adapter, Index 7 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC238650.14256714410679758TruSeq Adapter, Index 7 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGCCG193480.1155830339064873TruSeq Adapter, Index 7 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGCCG176140.10522428980922406TruSeq Adapter, Index 7 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1542200.044.26191
TCGGAAG1551850.043.904112
TATGCCG1522450.043.8211545
CGGAAGA1555350.043.7199753
ACACGTC1552500.043.387812
GCACACG1557100.043.32700710
CGTCTGA1552800.043.31567415
CACACGT1560100.043.24513611
ACGTCTG1554900.043.2441514
CACGTCT1557800.043.22285513
CTCGTAT1550450.042.85109341
CGTATGC1558550.042.83850543
TCGTATG1554400.042.80814442
TCTCGTA1456600.042.7789740
AGAGCAC1585000.042.753117
ATCTCGT1455100.042.73803739
CATCTCG1457850.042.67927638
GAAGAGC1591800.042.6439555
AGCACAC1585100.042.6354569
GAGCACA1588900.042.5717128