FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16498739
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGC6095173.6943247602134925TruSeq Adapter, Index 14 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG1245510.7549122390505117TruSeq Adapter, Index 14 (97% over 44bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTC283750.17198284062800193TruSeq Adapter, Index 14 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTATGC173970.10544442214644403TruSeq Adapter, Index 14 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA143500.043.508061
CGTATGC734050.041.68185445
ATCGGAA834150.037.3585621
TCGGAAG839900.037.0247732
CGGAAGA847300.036.6509633
TGCCGTC38750.036.12004545
ATGCCGT39150.035.63604444
ACACGTC872150.035.34045412
CGTCTGA873650.035.2488815
ACTCGTA25850.035.2474742
ACGTCTG874900.035.1856614
GCACACG879950.035.10048310
TCGTATG871550.035.04395344
CTCGTAT869650.035.04031443
CACGTCT879800.035.0306113
AGTTCCG872750.035.0266233
CACACGT882100.034.9996311
TTCCGTA871800.034.97984735
GTTCCGT874200.034.9299234
TCCGTAT826900.034.90957336
Produced by FastQC (version 0.11.5)