FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7139821
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGC331280.4639892232592386TruSeq Adapter, Index 25 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC40800.040.642345
ATCGGAA45150.037.4872131
TCGGAAG45750.036.8428232
TCTCGTA45000.036.6995442
TATCTCG45000.036.6495440
CTCGTAT44900.036.6309443
TCGTATG45500.036.44459544
ACACGTC45600.036.36416212
CGGAAGA47200.035.51833
ATCTCGT46450.035.45703541
CGTCTGA47050.035.33862715
ACGTCTG47450.034.89847614
CACGTCT48450.034.27153413
GCACACG49350.033.55533610
CACACGT50800.032.6861411
GATCGGA7150.029.2752061
GTCACAC56850.029.00998528
TCTGAAC60500.027.5939217
AGAGCAC61500.027.2553737
ACTGATA61950.026.69420633
Produced by FastQC (version 0.11.5)