FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8678499
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4300.021.45565445
ACGGCTA5050.017.37579231
CGGCTAC5050.017.37579232
CTCGTAT5450.017.33970341
ATCGGAA5950.017.035411
TCTCGTA5150.015.72838440
GTCACGG6500.014.884232528
CGTATGC6350.014.52894243
TCGGAAG7100.014.2600322
GATCGGA2601.7826096E-1013.8612911
ACACGTC7450.013.58994112
TCGTATG7350.013.16444342
ATCTCGT6500.013.1536639
AGTCACG7550.013.11209527
CGTCTGA7550.013.11194415
CGTTGGA22050.012.3604361
CGGAAGA8950.012.066523
ACGTCTG8500.011.91118414
TCACGGC8600.011.2497129
CATCTCG7250.011.17225538
Produced by FastQC (version 0.11.5)