FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18520901
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG915150.49411742981618445TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG98750.042.8996445
ATCGGAA110500.039.0921
CTCGTAT108700.038.9313741
TCTCGTA104200.038.82616840
CGTATGC109550.038.7525243
TCGGAAG112200.038.478682
TCGTATG110850.038.23715642
ACACGTC112300.038.02268612
ATCTCGT106750.037.91855239
CGGAAGA113800.037.8674963
CGTCTGA112800.037.83593815
ACGTCTG115750.036.96743814
CACGTCT118150.036.2352613
GCACACG120600.035.5177910
AATCTCG114350.035.3196838
CACACGT124000.034.70720311
GTATGCC123050.034.44606844
GTCACAC139600.030.60784328
GATCGGA11650.029.9801521
AGAGCAC147900.029.1747257
Produced by FastQC (version 0.11.5)