FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9308304
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12150.038.14553545
CTCGTAT13150.034.90873741
CGTATGC13750.034.04006643
TCGTATG13900.033.1870642
ATCGGAA14500.032.7489851
ACACGTC14450.032.54067212
TCGGAAG14900.031.8648552
TCTCGTA13100.031.77809340
CGTCTGA15200.030.49231115
ATCTCGT13800.029.6769839
CGGAAGA16600.028.7359053
CATCTCG14900.028.09015338
ACGTCTG17250.026.86859114
CACGTCT17550.026.40929813
GCACACG19400.024.58594310
CACACGT20250.022.88732111
GTATGCC20450.022.773544
CTTAGGC25550.018.49168432
ACTTAGG28950.016.24223731
AGAGCAC32950.015.1591897
Produced by FastQC (version 0.11.5)