FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10018982
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG437710.4368807130305254TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG61550.041.38601745
ATCGGAA65450.040.4776841
TCGGAAG66450.039.714132
CTCGTAT65050.039.05547741
CGTATGC65750.038.9819243
ACACGTC67250.038.70469312
TCGTATG66300.038.4888442
CGTCTGA68000.038.1787415
CGGAAGA69050.038.1524353
TCTCGTA55200.037.9529840
ACGTCTG69450.037.3816314
CACGTCT70300.037.08965713
TCACGAT55150.036.9586534
ATCTCGT57100.036.68972839
CATCACG68200.036.18671432
GCACACG73700.035.6842110
GTATGCC73250.035.144244
CACACGT74550.035.0655811
CACGATC57850.035.0392535
ACGATCT57900.034.93128236
Produced by FastQC (version 0.11.5)