FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678051
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC41840.24933687951081343TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5000.040.9548262
CGTATGC4900.040.8672944
GATCGGA5000.040.50961
TCTCGTA4950.040.45569641
ACCGATG5050.040.0989632
CGATGTA5050.039.65341634
TCGTATG5050.039.2078743
CTCGTAT5100.038.8246342
CACCGAT5300.038.63202331
CCGATGT5250.038.14280733
TCACCGA5500.037.22611230
TATCTCG5450.036.74416739
ATCTCGT5550.036.48752240
TCGGAAG5750.036.394513
ACACGTC5650.036.2388913
CGTCTGA5600.036.16066716
CACGTCT5800.035.30167814
ACGTCTG5950.034.41171615
GTCACCG5800.034.13686829
CGGAAGA6000.034.1239364
Produced by FastQC (version 0.11.5)