FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8053580
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGC253250.3144564280729812TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC30150.039.70037545
GATCGGA9000.033.7645651
TCGGAAG36800.032.4733852
ATCGGAA36850.032.3748861
GTCACGA36400.032.32698428
CTCGTAT36350.032.18616543
TCGTATG37300.031.96960844
TATCTCG36750.031.83583840
ACACGTC37450.031.66152212
TCTCGTA37000.031.6207342
CGGAAGA38250.031.3004283
ATCTCGT37550.031.09765841
ACGAGTG38000.030.96584931
AGTCACG38100.030.8255227
CACGAGT38250.030.8222830
CGTCTGA38550.030.69876315
ACGTCTG38950.030.383514
CACGTCT40250.029.51488313
GCACACG40250.029.40308410
CACACGT41400.028.69502311
Produced by FastQC (version 0.11.5)