FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5994409
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3500.027.64459445
CTCGTAT3850.025.13207843
TCTCGTA4350.022.76062242
TCGTATG4450.022.24877544
TATCTCG4900.020.2068740
ATCTCGT5100.019.85567941
ATCGGAA5050.019.1624981
ACACGTC5550.018.6453112
CGTCTGA5550.018.6453115
TCGGAAG5500.017.9991782
CGGAAGA7150.015.1042063
ACGTCTG6750.014.997313514
CACGTCT7250.014.58359513
GCACACG7900.013.38524210
CGTTGGA13750.011.7843281
ACGGGAT8950.011.0607649
CACACGT9650.010.72463111
CGGGATT9000.09.99937810
AAACGGG16000.09.8432197
AACGGGA11950.09.6019938
Produced by FastQC (version 0.11.5)