FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6050077
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG119770.1979644226015636TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15950.040.2069145
CGTATGC18600.034.47622743
CGATCAG18650.034.26116632
CTCGTAT18950.033.83862341
TCGTATG19200.033.3982942
TCTCGTA17400.033.1016640
GTCACGA19450.032.7362928
ATCGGAA19700.032.3529471
TCGGAAG20000.031.8547342
ACACGTC20150.031.70939812
CGTCTGA20400.031.65165515
ACGTCTG20350.031.50831414
ACGATCA20150.031.48739431
ATCTCGT18350.031.38794939
CGGAAGA20500.031.1881243
AGTCACG20750.030.79377427
TCACGAT20750.030.68534529
CACGTCT21850.029.34527213
GATCGGA4700.028.7506051
GCACACG22750.028.67858510
Produced by FastQC (version 0.11.5)