FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13696035
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10450.031.4810121
CGTATGC13200.024.03213944
CTCGTAT13700.023.15598342
ATCGGAA15000.022.0563432
TCTCGTA12700.021.79044341
TCGTATG14950.021.67032243
TCGGAAG16150.020.6251263
ACACGTC16000.020.52883313
CGTCTGA16900.019.30274416
TATGCCG5700.018.945945
ATCTCGT15500.017.99926240
ACGTCTG19350.016.85866515
CGGAAGA19850.016.7807674
CACGTCT22250.014.96453114
GCACACG22200.014.795608511
GTATGCC23950.013.62107345
AATCTCG21350.013.38362339
CACACGT25500.013.14546312
CGTTGGA30700.011.8901891
CGGGATT19450.010.98918610
Produced by FastQC (version 0.11.5)