FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13373906
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12650.034.32379545
CTCGTAT16650.026.07783741
TCGTATG16700.025.86504642
CGTATGC17150.025.1863743
ATCGGAA17700.025.152951
TCTCGTA15850.024.12949240
TCGGAAG19000.023.7994942
ATCTCGT16500.023.1787639
ACACGTC19050.023.0284612
CGTCTGA19200.022.73137915
CGGAAGA22300.020.176713
ACGTCTG21900.020.03160514
CATCTCG20000.019.57241838
GATCGGA6000.018.8327921
CACGTCT25000.017.72766113
GCACACG25200.017.40841910
GTATGCC27200.016.29392844
CACACGT27950.015.85658611
CTTAGGC33900.013.27491932
CGTTGGA31350.011.750221
Produced by FastQC (version 0.11.5)