FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4701041
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG52730.11216664564295441TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7150.038.0221845
ATCGGAA8350.032.0299871
CGTATGC8550.031.27076743
TCGGAAG8700.030.9948062
CTCGTAT8800.030.6373841
TCGTATG9050.029.7910442
CGTCTGA9300.029.7152815
TCTCGTA7550.029.43189840
ACACGTC9350.028.59519412
ATCTCGT8250.026.93464739
CGGAAGA10200.026.4364663
ACGTCTG10450.025.80017314
GATCGGA2150.024.0428331
CACGTCT11350.023.5563913
GCACACG11850.022.94140810
CACACGT12700.021.22927511
GTATGCC13100.021.0955844
GTCACAC15800.017.20660428
AGAGCAC17200.016.1974357
GATCTCG13800.015.93792538
Produced by FastQC (version 0.11.5)