FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10639052
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGC154370.14509751432740436TruSeq Adapter, Index 27 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22300.037.6327345
CTCGTAT22900.036.3577843
ATCGGAA23500.036.2938541
TCGGAAG24450.035.0634652
TCGTATG23850.034.7153244
TCTCGTA21750.034.3486742
ACACGTC24650.034.3180412
TATCTCG22050.033.982640
ATCTCGT23200.032.0075941
CGTCTGA26650.031.74392115
CGGAAGA27400.031.2873543
ACGTCTG27600.030.81432214
GCACACG29800.028.99121910
CACGTCT29600.028.73227313
CACACGT31550.026.95528811
AGAGCAC46250.019.4597197
GAAGAGC49650.017.9912595
AGCACAC48900.017.9435049
TCTGAAC49350.017.46149817
GAGCACA51100.017.3045358
Produced by FastQC (version 0.11.5)