FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10023709
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC207750.2072586105602228TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA26400.039.397931
TATGCCG9900.032.2721945
CGTATGC33550.030.71422844
CTCGTAT33400.030.58423842
TCTCGTA34400.029.76057241
ACACGTC35400.029.61593413
TATCTCG34300.029.58523839
TCGTATG34900.029.52761343
ATCGGAA35950.029.2935542
CGTCTGA36400.028.67869416
ATCTCGT35800.028.408240
TCGGAAG37650.027.851343
ACGTCTG37950.027.44808215
CGGAAGA38600.027.2824674
CACGTCT38900.026.83559214
GCACACG41350.025.57253511
GTATGCC41500.024.88512845
CACACGT42600.024.87448712
CACTAGC43550.023.71668431
GTCACTA45850.022.47621229
Produced by FastQC (version 0.11.5)