FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9328502
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9400.032.31305345
CTCGTAT10200.029.33752841
TCTCGTA10350.028.04295540
ATCGGAA12450.027.8488661
ACACGTC12500.027.35860812
CGTATGC11150.027.24149743
CGTCGAA1450.026.37782915
TCGTATG11650.026.07233442
TCGGAAG13250.025.8177592
ACGTCGA1400.025.71284114
CACGTCG1500.025.49870313
CGTCTGA12500.024.47862415
CGGAAGA14600.023.8921763
ATCTCGT12450.023.13209739
ACGTCTG14150.021.94222814
CACGTCT14900.021.29086313
GTCGAAC1800.019.99887516
GCACACG18700.019.13004310
AATCTCG16150.018.80779838
CACACGT18650.018.57814411
Produced by FastQC (version 0.11.5)