FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4471872
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG61620.13779464170709715TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8250.038.6712645
GATCGGA4550.030.6235561
CTCGTAT11050.028.66920341
CGTATGC11350.027.91111643
GTCACGA11300.027.83578728
TCGTATG11650.027.19267842
ACGATCA11650.026.9995231
TCTCGTA10000.026.98397840
CGATCAG11800.026.84670632
CGTCTGA11850.026.54412815
AGTCACG11950.026.5097227
ACACGTC12050.026.4764712
TCACGAT12050.026.47617329
ACGTCTG12500.025.7030614
ATCGGAA12550.025.2493781
TCGGAAG12650.025.045582
ATCTCGT11050.024.4198939
CGGAAGA13500.023.4678463
CACGTCT13900.023.1142613
GCACACG14550.022.3902510
Produced by FastQC (version 0.11.5)