FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26400881
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC30650.032.9594345
CTCGTAT31500.031.7843543
TCGTATG32700.030.54914744
ATCGGAA33400.030.3503171
TCTCGTA29800.029.52054442
CGTCTGA35300.028.74386415
ACACGTC35500.028.51941512
TCGGAAG35600.028.3290582
ATCTCGT33100.026.78127941
CGGAAGA39100.026.0810783
ACGTCTG42750.023.89330514
CACGTCT44400.023.25874113
GCACACG49550.020.8416510
AATCTCG44900.019.89331840
CACACGT53150.019.38743211
AGTCAAC73150.014.33240233
GTCAACA82000.012.75811634
AGAGCAC85200.012.3583197
AGCACAC89800.011.8006969
CGTTGGA56200.011.5840041
Produced by FastQC (version 0.11.5)