FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122258
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG246911.1634306479231082TruSeq Adapter, Index 20 (97% over 44bp)
CCCAAAACCTTATCTAATAATAACCAAAACCATCCACCACCACCTTCCAAT44720.21071896065417117No Hit
GAAGAAGAGGAGGGAGGTTGTTGTGTGTTGTGTTGGGGTTGTTTAGATGTT40720.19187111086399486No Hit
ACTTTAAAAAAAACTCCTAACATCAAAAACTAACTCTCCACAACTAACAAA23710.11172062963127008No Hit
GGGGAAGTTTAGAATTTTTTGGTGGGAATGTTGGTTTTGTGGTGTGGATTG22520.10611339431869264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA207.0306467E-445.00518443
TACGGCG1750.043.7121126
GATCGGA29950.042.9398841
AGTCACG31050.042.75250228
CGTCTGA32300.042.69911216
CGTGGCC29650.042.56955333
ATCGGAA30800.042.514122
ACGTGGC29850.042.51044532
ACACGTC32800.042.45977813
GTCACGT30350.042.25489829
ACGTCTG32900.042.0571815
TCGGAAG31350.041.9835553
CACGTCT33450.041.5001814
ACGGCGA1900.041.445317
TCTCGTA31000.041.37573243
TATCTCG30800.041.35021641
CTCGTAT30950.041.29716544
TCACGTG31150.041.169730
CACACGT33950.041.08779512
CGGAAGA32850.041.0253524
Produced by FastQC (version 0.11.5)