FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005310664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15821318
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG12867588.133064514599859TruSeq Adapter, Index 11 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC405630.25638192721996994TruSeq Adapter, Index 11 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAACTCGTATGCCG391670.24755838925682425TruSeq Adapter, Index 11 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACACCTCGTATGCCG172520.10904274852449082TruSeq Adapter, Index 11 (98% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)