FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4732279
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3150.024.9639445
TCTCGTA3000.024.71482342
ATCGGAA3550.023.4260391
TCGTATG3450.022.79316544
CTCGTAT3500.021.82607843
TCGGAAG3950.021.049112
ACACGTC3800.020.69379412
CGTCTGA4050.019.97115315
ATCTCGT4150.017.86613741
CGGAAGA4900.017.4258373
ACGTCTG4900.016.9652914
CCCGTAA1502.605957E-614.9842231
CACGTCT5250.014.97836413
AATCTCG5550.013.48158540
GCACACG6150.013.15159610
CCTCGAA2706.369555E-811.6543951
CACACGT7350.011.61587511
CGGGATT6800.011.56405710
CCCGGTT1953.899807E-511.5237672
ACGGGAT7150.011.3122129
Produced by FastQC (version 0.11.5)