FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11265778
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5450.016.51603145
ATCGGAA7400.013.7375571
CGTCTGA8400.013.65877215
GATCGGA4350.013.5024571
CGTATGC7300.013.25524743
CTCGTAT7600.012.7320741
TCGTATG7950.012.45454342
TCGGAAG8000.012.3736262
ACACGTC8750.011.56973412
TCTCGTA7300.010.4809440
ACGTCTG10350.010.43329914
ACCGCGC9050.010.19201331
CGGAAGA10900.09.70054153
CGTTGGA22150.09.68899251
CACCGCG10250.09.6572530
CACGTCT12200.09.40440113
ACGGGAT16000.08.99896059
ATCTCGT8750.08.74386639
AAACGGG25550.08.7171157
CCGAGTA20750.08.5653132
Produced by FastQC (version 0.11.5)