FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9839352
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8800.026.33449245
GATCGGA6750.025.3466681
CGTTTCG11100.020.47469132
TCTCGTA12200.018.8114342
CTCGTAT12300.018.29263943
GTTTCGG12450.018.25463533
TCGGAAT11700.017.88420736
ATCGGAA13250.017.3298721
TTCGGAA12650.016.71898535
TCGTATG13450.016.56045544
ATCTCGT14100.016.11690141
GTCACGT13950.015.48509228
TCGGAAG14550.015.4656882
CGTCTGA14800.015.049643515
CGGAATC14700.015.00026837
TTTCGGA15250.014.75421134
ACACGTC14800.014.7456112
AGTCACG15400.013.88075327
CGGAAGA16900.013.7143763
TCACGTT16600.013.69049129
Produced by FastQC (version 0.11.5)