FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9048206
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG305340.3374591604125724TruSeq Adapter, Index 22 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGC112600.12444455840196389TruSeq Adapter, Index 22 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA37200.041.877151
CGTATGC19050.036.4955445
CGTACGT51500.029.70929734
CCGTACG51500.029.66773833
ACCGTAC52550.029.16058732
GTACGTA53350.028.8468235
ATCGGAA54150.028.8382232
TCTCGTA52650.028.7183343
CGTAATC52950.028.63965638
CTCGTAT53150.028.53157244
GTCACCG53800.028.52347229
TCGTATG53950.028.35918445
ACACGTC54450.028.30381413
TCGGAAG55250.028.1417333
ACGTAAT54300.028.136237
TACGTAA55100.027.889836
ATCTCGT54400.027.83584842
CACCGTA55400.027.70106531
CGTCTGA56100.027.4713516
CGGAAGA56550.027.4550084
Produced by FastQC (version 0.11.5)