FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9533480
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6750.029.708021
CGTATGC7850.026.36781944
TCTCGTA6700.024.51394541
CTCGTAT8300.024.39661442
TCGTATG9000.022.49839443
ACACGTC9300.022.4973313
ATCGGAA9500.021.321762
TCGGAAG9350.021.18243
CGTCTGA10100.020.9384416
ACGTCTG10750.019.67239615
CGGAAGA10950.018.9096034
TATGCCG1951.8189894E-1218.46041345
ATCTCGT8950.018.35122140
CATCTCG10050.016.11884139
GCACACG13550.015.77303611
CACGTCT13250.015.62078914
GTATGCC13650.015.32873545
CACACGT16400.012.75763312
CGTTGGA20400.011.5970261
AACGGGA19350.010.3484248
Produced by FastQC (version 0.11.5)