FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9012939
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT12050.040.1452133
CCGTACG12200.039.83604432
ACCGTAC13600.035.73527531
CTCGTAT13800.035.70749343
GTACGTA13800.034.8914834
CGTATGC14500.034.60439345
TCGTATG14500.033.98368544
ATCGGAA15000.033.788411
TCGGAAG15150.033.5630382
TCTCGTA12950.033.5337342
ACACGTC15250.032.89979612
CGTAATC13400.031.90332237
CGTCTGA15500.031.64391715
TACGTAA15350.031.51481835
CACCGTA15550.031.39869930
GTCACCG15450.031.16468628
ACGTAAT13750.031.09123636
ATCTCGT14350.030.4187841
CGGAAGA17100.029.7353423
ACGTCTG17550.028.07581914
Produced by FastQC (version 0.11.5)