FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17020894
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6550.016.83255845
CTCGTAT8500.013.76527443
CGTTGGA44500.013.5620381
TCTCGTA8550.013.42160642
CCGCGAT1454.8520952E-412.4145612
TCGTATG9150.011.80376644
ACGGGAT31600.011.7476269
AAACGGG49900.011.5436647
ATCGGAA9850.011.20237351
AACGGGA41850.011.1826668
GAAACGG51600.010.9019016
GTCACGT11800.010.86842228
ACACGTC10900.010.73318912
GGAAACG53100.010.3820285
CGGGATT29750.010.28502110
AACGATC11800.09.72417737
ATCTCGT11550.09.5458241
CGTCTGA13000.09.17245815
AGTCACG12900.09.06958227
CGGGATA8200.09.05426710
Produced by FastQC (version 0.11.5)