FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8307882
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG487840.5872014070493539TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60200.043.23988345
ATCGGAA63450.042.1002241
TCGGAAG64050.041.700572
ACACGTC63550.041.5975112
CTCGTAT62500.041.5846741
TCGTATG63000.041.3260742
CGTATGC63200.041.19529343
TCTCGTA56150.041.07738540
CGTCTGA64650.040.78804415
CGGAAGA65650.040.6487583
ATCTCGT56750.040.20691739
ACGTCTG65650.040.09820614
CACGTCT66250.039.83693713
GCACACG67800.039.15591410
CATCTCG59200.038.61897338
GTATGCC68050.038.41719444
CACACGT69800.037.9372711
AGTCACC77200.033.89606527
GATCATC68400.033.4510635
CCAGATC78600.033.2604932
Produced by FastQC (version 0.11.5)