FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7339232
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG185600.2528874955853691TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25050.041.22574245
CGATCAG27100.037.77381532
ATCGGAA27900.037.509351
CTCGTAT27550.037.4906241
GTCACGA27550.037.3242128
CGTATGC27750.037.3017643
TCGTATG27850.037.0062342
TCTCGTA24300.036.8555140
TCGGAAG28550.036.6488762
ACGATCA28500.035.83931431
TCACGAT28700.035.7529
CGTCTGA29150.035.6580915
AGTCACG29050.035.16460827
ATCTCGT25250.035.0232839
ACACGTC29800.034.87983712
CGGAAGA30300.034.5310133
ACGTCTG30800.033.74783314
CACGTCT31450.033.19341713
CACGATC32050.032.3642830
GCACACG32400.032.28959310
Produced by FastQC (version 0.11.5)