FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9989112
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC135850.13599807470373743TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG64000.042.5400245
GATCGGA16650.037.566911
ATCGGAA80450.034.7352521
CGTATGC78650.034.64546643
ACACGTC80350.034.63829812
CTCGTAT78400.034.5837441
CGTCTGA79700.034.5255715
TCGGAAG80900.034.5137062
TCGTATG79200.034.4332842
TCTCGTA78600.034.38123340
ATCTCGT79450.033.98457339
ACGTCTG81150.033.9641114
CGGAAGA82750.033.823673
CACGTCT83650.033.2987113
GCACACG84650.033.11764510
AATCTCG84500.031.953738
CACACGT87800.031.8785511
GTATGCC85950.031.754844
GTCACAC94600.029.2790528
AGAGCAC100700.027.9732697
Produced by FastQC (version 0.11.5)