FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872351
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG128030.44573243311837585TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG16200.042.9133833
GATCGGA17700.042.4891361
ATCGGAA18550.041.965172
TCGGAAT16400.041.70412437
CGTCTGA19100.041.5813116
GTTTCGG16750.041.5042834
TTCGGAA16550.041.32613836
GTCACGT17200.041.2032429
TCGGAAG18900.041.1880383
ACACGTC19550.041.0838113
AGTCACG17950.040.73645828
CGGAAGA20100.040.1841854
TTTCGGA17350.039.93930435
ACGTTTC17550.039.86874432
ACGTCTG20300.039.1226215
CACGTCT20400.039.0411314
CGGAATC17450.038.93684438
TCACGTT18250.038.8326430
GCACACG21100.038.8121911
AATCTCG17750.038.53695341
Produced by FastQC (version 0.11.5)