FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9745683
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1143191.1730219421255543TruSeq Adapter, Index 1 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG106320.10909445751518902TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA126700.044.152431
TCGGAAG142300.039.3601153
ATCGGAA142300.039.3595122
ACACGTC142500.038.87685413
TCTCGTA141050.038.8379641
CTCGTAT141400.038.8023542
CGTCTGA142850.038.79932816
ATCTCGT141600.038.70380840
CGGAAGA144500.038.651944
TCGTATG143050.038.465443
TCACGAT143000.038.4211335
CGTATGC143250.038.40669344
ACGTCTG145200.038.1402115
CACGTCT145850.037.983914
ACGATCT144300.037.948437
CATCACG145050.037.87812433
CACGATC145100.037.75644336
GCACACG147350.037.6584611
CACACGT149000.037.2261512
GTATGCC150000.036.72672345