FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10409794
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG7750.026.9831732
CGTATGC8350.026.65963445
CTCGTAT8950.023.36497743
TCTCGTA9750.021.67847342
GTTTCGG9800.021.5684933
TTCGGAA10500.020.77285635
TCGTATG10500.020.1300144
TTTCGGA10700.019.9644734
ATCGGAA10900.019.7491361
TCGGAAT10700.019.54388636
TCGGAAG10700.019.5434152
GTCACGT11100.019.24493828
ATCTCGT11200.019.0726541
ACACGTC11350.018.82040812
AGTCACG10800.018.73831227
CGTCTGA11400.018.34338215
GATCGGA3450.016.773071
CGGAATC12950.016.32186537
ACGTCTG13250.015.95193114
CGGAAGA14200.015.3597693
Produced by FastQC (version 0.11.5)