FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6035579
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5850.032.26144443
TCTCGTA5600.029.68982542
CGTATGC6550.028.81341745
ATCGGAA7100.026.9076461
TCGTATG7200.026.83630444
TCGGAAG7200.026.5282122
ACACGTC7300.026.1609112
ATCTCGT6650.024.6638941
CGTCTGA7800.023.04369715
CGGAAGA8550.022.8648013
CACGTCT9400.020.07743513
ACGTCTG9200.020.02547314
GCACACG10950.018.26118910
AATCTCG9700.016.59632340
CACACGT11700.016.51464811
ACATGTC18200.011.60405131
GAGCACA18550.011.1428718
CGTTGGA13300.010.8154211
CAGTCAC20100.09.94850726
CCAGTCA20400.09.91234325
Produced by FastQC (version 0.11.5)