FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10319495
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC503400.487814568445452TruSeq Adapter, Index 7 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG357550.34648013299100394TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA55250.042.9754831
TATGCCG50650.041.3131545
TCGCGTA608.196721E-626.25128440
TACGCCG651.5252201E-524.23089845
CTCGTAT101750.022.77763642
ACACGTC102500.022.60779413
CGCGTAT500.002265118522.5012141
CGTCTGA103100.022.4778616
TCGTATG103050.022.46812843
TCTCGTA102600.022.45734841
CGTATGC103100.022.45701644
ATCGGAA106100.022.3123972
TCGGAAG105550.022.2793123
ACGTCTG104300.022.15313315
ATCTCGT104400.022.13470340
CGGAAGA107000.022.0615224
CATCTCG105500.021.81849539
CACGTCT107250.021.62770314
GTATGCC108900.021.26044345
GCACACG110000.021.14816511
Produced by FastQC (version 0.11.5)