FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6258899
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG103750.16576397861668643TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA13450.040.324661
TCTCGTA17850.029.62467243
CTCGTAT18400.028.86006444
AACGATC18600.028.54951538
ATCGGAA19100.028.2740442
TCGGAAG19550.027.8534283
GTCACGT19250.027.81816729
TCGTATG19250.027.58484545
ATCTCGT19300.027.28239442
CGTATGC6600.027.2731545
AGTCACG19800.027.15929428
CGTCTGA19900.027.02238316
CGGAAGA20400.026.3619634
ACACGTC20600.025.88301313
AAACGAT21350.025.18834737
ACGTCTG21500.025.1138615
ACGATCT21100.025.06103339
CACGTGA21800.024.9770531
TGAAACG21850.024.7129635
ACGTGAA21950.024.7038632
Produced by FastQC (version 0.11.5)