FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7606801
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6950.033.34538345
CGTATGC8200.028.26224543
TCTCGTA8350.027.48471640
CTCGTAT8750.026.22827141
TCGTATG8850.025.9319142
ATCGGAA9200.025.4503861
ATCTCGT8950.025.39077239
TCGGAAG10200.023.166832
ACACGTC10400.022.71489112
CGTCTGA10850.022.18752115
CATCTCG10350.022.1736638
ACGTCTG11000.022.08949714
CGGAAGA11600.020.1768253
CACGTCT12200.019.73234613
GCACACG13750.017.83522210
CACACGT14000.017.51673711
GTATGCC14300.016.36366744
CGTTGGA19850.013.8371981
CTTAGGC18100.013.42490932
ACGGGAT13600.012.9035869
Produced by FastQC (version 0.11.5)