FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5643254
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGC63590.1126832143298884TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8250.035.67609443
CGTATGC8400.034.7712445
TATCTCG7900.034.72991640
TCTCGTA7900.034.69706342
AGTTCCG8500.034.62618633
ACACGTC8900.034.3324712
ATCGGAA8850.033.7797931
TCGTATG8750.033.3803944
GTTCCGT9150.033.2066734
CCGTATC8400.032.932637
TCGGAAG9800.031.1841852
CGTCTGA9450.031.14552315
TTCCGTA9400.031.11741335
TCCGTAT8950.030.65518636
ATCTCGT9200.030.03816441
CGTATCT9700.028.51895338
ACGTCTG10900.028.23905814
CGGAAGA11000.027.5779913
CACGTCT11450.025.9014813
GCACACG12050.024.79801410
Produced by FastQC (version 0.11.5)