FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12748897
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11900.029.11734245
CCGTACG12900.027.38311232
GATCGGA7150.026.755531
CGTACGT13200.026.07886533
ACCGTAC14450.024.29002231
CTCGTAT14900.022.35112843
GTACGTA15650.021.99579834
TCTCGTA15450.021.55570842
GTCACCG17100.020.13105228
TCGTATG16600.020.06160544
ATCGGAA18650.020.0322591
CGTAATC17350.019.97191237
TCGGAAG18900.019.64552
CACCGTA18600.019.59641530
CGTCTGA18100.019.01742215
ACGTAAT18650.018.94178636
ATCTCGT18150.018.84492141
ACACGTC18850.018.38004112
CGGAAGA20400.017.9803623
TACGTAA19400.017.9759835
Produced by FastQC (version 0.11.5)