FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29792857
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG2533180.8502642093035924TruSeq Adapter, Index 8 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGCCG308920.10368928364272013TruSeq Adapter, Index 8 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG350500.043.59976645
ATCGGAA369900.043.1408921
TCGGAAG373000.042.6151242
CGTATGC361300.042.4210143
CTCGTAT349600.042.3862241
ACACGTC374250.042.25052612
CGTCTGA374350.042.11283515
TCGTATG362700.042.0957942
TCTCGTA295800.041.9746840
CGGAAGA378350.041.9232753
ATCTCGT300100.041.44820439
ACGTCTG381350.041.440114
CACGTCT383050.041.34448613
GCACACG389200.040.72007410
CACACGT393250.040.28926511
GTATGCC383350.040.09835444
AATCTCG313300.039.95309438
GTCACAC413350.037.2422228
ACTCGTA44400.037.24170340
AGAGCAC435950.036.590747
Produced by FastQC (version 0.11.5)