FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9730516
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8750.033.9569361
ATCGGAA12500.024.485372
CTCGTAT12250.024.24423642
TCGTATG12600.023.57078643
TCTCGTA12600.023.39221841
CGTATGC12800.023.20249244
TCGGAAG13250.022.7588923
ACACGTC13750.021.92678613
ATCTCGT14150.020.98882740
CGTCTGA15200.019.98259516
TATGCCG5100.019.85219645
CATCTCG16200.018.61060339
CGGAAGA16350.018.581394
ACGTCTG16700.017.91830415
CACGTCT17600.017.00202814
GCACACG19300.015.97114811
GTATGCC19650.015.45743445
CACACGT19900.015.03735612
CGTTGGA21950.012.1006891
GAGCATC26450.012.07900336
Produced by FastQC (version 0.11.5)