FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10044632
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG119150.11862057266010342TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15100.037.8481445
TCTCGTA17300.032.90666240
ATCGGAA18250.032.2034841
CGTATGC18100.031.82517643
CTCGTAT18100.031.57654241
ACACGTC18950.030.86893712
TCGTATG18700.030.44306242
TCGGAAG19300.030.3120022
ATCTCGT18900.030.12091339
CGTCTGA20100.029.32594115
TCACGAT19800.029.20562734
CGGAAGA20500.028.647263
ACGTCTG20950.027.92132814
CATCACG22600.026.18342632
CACGTCT22950.025.58676513
CACGATC23100.024.83858335
GCACACG23750.024.81974810
ACGATCT23550.024.07732436
CACACGT25900.023.02002511
GTATGCC25700.022.93916544
Produced by FastQC (version 0.11.5)