FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370336
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC81070.240539815614823TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9250.041.1176641
TCACGAT9250.039.89051435
ACACGTC10000.039.14922713
CGTATGC9250.038.9181544
CTCGTAT9250.038.9181542
ATCGGAA10000.038.257192
CGTCTGA10050.038.06120716
ATCTCGT9750.037.6146440
TCTCGTA9800.036.73396741
TCGTATG9900.036.59018343
TCGGAAG10550.036.4755063
CGGAAGA10550.036.255744
CATCACG10550.035.40161533
CACGTCT11250.034.80137614
ACGATCT10550.034.76182637
ACGTCTG11100.034.66353615
CACGATC10600.034.5978636
CACACGT11550.033.89543512
GCACACG11750.032.74354611
GTATGCC11600.031.42179145
Produced by FastQC (version 0.11.5)