FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10726458
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG272270.25383029514495836TruSeq Adapter, Index 16 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGC159010.14824091978917925TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA33000.038.663171
CGTATGC24600.035.58003645
CGTCCCG49300.024.87318235
GTCCCGA49550.024.79297836
TCCCGAT49800.024.71369637
CCCGATC49750.024.64808338
CCGATCT49800.024.48779339
CTCGTAT50900.024.1360844
ACCCGTC51900.023.75718332
ATCGGAA54000.023.7081812
TCTCGTA52100.023.53697643
TCGTATG52100.023.4937945
TCGGAAG54800.023.4031333
TCACCCG52700.023.35384830
ACACGTC54450.023.30563513
CACCCGT53450.023.11034231
ATCTCGT53700.023.04486842
CCCGTCC53350.023.02713833
CGTCTGA55050.023.01075216
CCGTCCC53450.022.89986634
Produced by FastQC (version 0.11.5)