FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15869859
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG166140.10468902086653699TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23150.037.12204445
CTCGTAT26700.031.9336741
ATCGGAA28050.031.4542061
CGTATGC27900.030.7213543
TCGTATG28450.030.28606442
TCTCGTA27050.030.19008440
TCGGAAG29900.029.3590182
CGTCTGA30450.028.58965715
ACACGTC30650.028.54979912
CGGAAGA31250.028.3788193
ATCTCGT29500.027.83520739
ACGTCTG32450.026.82758314
CACGTCT34550.025.58753413
GCACACG38500.022.96318610
GTATGCC39800.021.87496644
CACACGT41400.021.57139411
GATCGGA6400.021.2089061
GATCTCG46750.017.66070638
GTCACAC53900.016.52821528
AGAGCAC62800.014.471837
Produced by FastQC (version 0.11.5)