FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24162883
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19950.032.8140945
CTCGTAT22950.027.64251941
CGTATGC24250.027.18110343
TCGTATG24200.027.14430242
ATCGGAA25900.026.5102421
TCGGAAG26950.025.6362972
ACACGTC27100.025.31798712
TCTCGTA21750.024.51412840
CGTCTGA28350.024.20157215
CGGAAGA29500.023.4106333
ATCTCGT24400.022.2205439
TCACGAT25600.022.05783834
ACGTCTG31900.021.57881414
CATCACG33500.020.348532
CACGTCT34900.020.11072713
GCACACG39250.018.16846510
CACACGT42050.016.74468411
GTATGCC42300.015.90164444
CACGATC34700.015.689713535
ACGATCT34900.015.53524436
Produced by FastQC (version 0.11.5)