FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12199103
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG247390.2027935988408328TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29700.040.3780445
CGTATGC35000.034.5202243
TCTCGTA34950.034.05362740
CTCGTAT35100.033.97233641
TCGTATG35800.033.56014642
ATCGGAA36100.033.552141
TCGGAAG37500.032.1611182
ACACGTC37800.032.1406812
CGTCTGA37750.032.00445615
ATCTCGT37250.031.8905939
ACGTCTG39400.030.66416714
CGGAAGA40450.030.149263
CACGTCT41400.029.45452913
AATCTCG41450.028.76777338
GCACACG42950.028.28841810
GTATGCC45150.026.90935944
CACACGT45800.026.38073311
GATCGGA7300.023.7472341
AGTCACC58200.020.83912327
GTCACCT59100.020.3694828
Produced by FastQC (version 0.11.5)